The indoor air with different air pollutants is a significant health concern. As indoor environments typically consists of a complex mixture of chemical emissions from building materials and cleaning agents, microbial toxins etc., it is challenging to identify and solve the problems of unhealthy indoor air.
FICAM has in co-operation with SEA Oy (www.sisailmatutkimuspalvelut.fi) developed a rapid screening test to identify indoor air toxicity. The test recognizes potential health hazards of the indoor air regardless of their source. Additional investigations concerning the mechanisms behind the detected air toxicity can then be employed.
Principle of the test method: The condensed water indoor air samples are collected using the E-collector developed by SEA Oy. The samples are dosed to human THP-1-derived macrophages, incubated for 24 hrs, after which the macrophage viability is investigated using WST-1 salt. Living cells metabolize WST-1 to a colored dye, whose absorbance is then measured. Absorbance is directly proportional to the amount of living cells. Results are given as percentage inhibition of viability of indoor air sample treated cells compared to untreated control cells.