FICAM has developed and validated a standardized in vitro vasculogenesis/angiogenesis model. It is based on a coculture of human HUVEC (umbilical vein endothelial) cells and hASC (adipose stromal) cells. A dense network of tubules (blood vessels) is formed when the culture is induced in a specific way. The model is in routine use mainly to study to the effects of chemicals on the formation of blood vessels.
To extend the use of the vasculogenesis/angiogenesis model, further applications are being developed:
- addition of other cell types to study e.g. cell-cell interactions and testing effects of cellular components
- combining of metabolizing component (e.g. hepatocytes) to the model
- use of the model to study vascular-system related disease mechanisms e.g pre eclampsia ( 1 Virtanen et al. 2016)
- transformation of the standard model to a specific disease model e.g. by using transfected disease specific cells
1 Virtanen A , Toimela T, Tihtonen K, Sarkanen JR, Huttala O, Heinonen T, Uotila J (2016): Strong inhibitory effect of pre-eclampsia serum on angiogenesis detected in vitro by human cell-based angiogenesis tests. Pregnancy Hypertens 6(4):367-373.