Immunopharmacological Research Group

Publications focusing on natural products:

H. Eräsalo, M. Hämäläinen, T. Leppänen, I. Mäki-Opas, M. Laavola, R. Haavikko, J. Yli-
Kauhaluoma, E. Moilanen. Natural stilbenoids have anti-inflammatory properties and
down-regulate the expression of inflammatory mediators NO, IL6 and MCP1 possibly
in a PI3K/Akt-dependent manner. Submitted for publication.

I. Mäki-Opas, M. Hämäläinen, L. Moilanen, R. Haavikko, T.J. Ahonen, S. Alakurtti, V.M.
Moreira, K. Muraki, J. Yli-Kauhaluoma, E. Moilanen. Pyrazine-fused triterpenoids block
TRPA1 ion channel in vitro and inhibit TRPA1-mediated acute inflammation in vivo.
Submitted for publication.

M. Laavola, T. Leppänen, H. Eräsalo, M. Hämäläinen, R. Nieminen, E. Moilanen: Anti-
inflammatory effects of nortrachelogenin in murine J774 macrophages and in
carrageenan-induced paw edema model in the mouse.
Planta Med 83(6):519-526, 2017.

R. Puupponen-Pimiä, L. Nohynek, R. Juvonen, T. Kössö, P. Truchado, B. Westerlund-
Wikström, T. Leppänen, E. Moilanen, K.-M. Oksman-Caldentey: Fermentation and dry
fractionation increase bioactivity of cloudberry (Rubus chamaemorus).
Food Chem197:950-958, 2016.

L.J. Moilanen, M. Hämäläinen, L. Lehtimäki, R.M. Nieminen, K. Muraki, E. Moilanen:
Pinosylvin inhibits TRPA1-induced calcium influx in vitro and TRPA1-mediated acute
paw inflammation in vivo.
Basic Clin Pharmacol Toxicol 18:238-242, 2016.

M. Laavola, R. Haavikko, M. Hämäläinen, T. Leppänen, R. Nieminen, S. Alakurtti, V.
Moreira, J. Yli-Kauhaluoma, E. Moilanen: Betulin derivatives effectively suppress
inflammation in vitro and in vivo.
J Nat Prod 79:274-280, 2016.

Timonen, K. Vuolteenaho, T. Leppänen, R. Nieminen, E. Moilanen, P. Aulaskari, J. Jänis:
7-(2-Oxoalkoxy)coumarins – Synthesis and anti-inflammatory activity of a series of
substituted coumarins.
J Het Chem 52:1273–1295, 2015.

M. Laavola, R. Nieminen, T. Leppänen, C. Eckerman, B. Holmbom, E. Moilanen:
Pinosylvin and monomethyl pinosylvin, constituents of extract from the knot of pinus
sylvestris, reduce inflammatory gene expression and inflammatory response in vivo.
J Agric Food Chem 63:3445-3453, 2015.

R. Puupponen-Pimiä, T. Seppänen-Laakso, J. Maukonen, R. Törrönen, M. Kolehmainen,
M. Kankainen, T. Leppänen, E. Moilanen: Effects of ellagitannin rich berries on blood
lipid profiles, gut microbiota and metabolism of phenolic compounds in metabolic
DOSIS 33:55-76, 2015.

R. Puupponen-Pimiä, T. Seppänen-Laakso, M. Kankainen, J. Maukonen, R. Törrönen, M.
Kolehmainen, T. Leppänen, E. Moilanen, L. Nohynek, A.-M. Aura, K. Poutanen, F.A.
Tómas-Barberán, J.C. Espin, K.-M. Oksman-Caldentey: Effects of ellagitannin-rich
berries on blood lipids, gut microbiota and urolithin production in human subjects with
symptoms of metabolic syndrome.
Mol Nutr Food Res 57:2258-2263, 2013.

M. Laavola, R. Nieminen, M.F. Yam, A. Sadikun, M.Z. Asmawi, R. Basir, J. Welling, H.
Vapaatalo, R. Korhonen, E. Moilanen: Flavonoids eupatorin and sinensetin present in
Orthosiphon stamineus leaves inhibit inflammatory gene expression and STAT1
Planta Med 78:779-786, 2012.

M. Kolehmainen, O. Mykkänen, P. Kirjavainen, T. Leppänen, E. Moilanen, M. Adriaens,
D. Laaksonen, M. Hallikainen, R. Puupponen-Pimiä, L. Pulkkinen, H. Mykkänen, H.
Gylling, K. Poutanen, R. Törrönen: Bilberries reduce low-grade inflammation in
individuals with features of metabolic syndrome.
Mol Nutr Food Res 56:1501-1510,2012.

J.M. Timonen, R. Nieminen, O. Sareila, A. Goulas, L.J. Moilanen, M. Haukka, P.
Vainiotalo, E. Moilanen, P.H. Aulaskari: Synthesis and anti-inflammatory effects of a
series of novel 7-hydroxycoumarin derivatives.
Eur J Med Chem 46:3845-3850, 2011.

M. Hämäläinen, R. Nieminen, M.Z. Asmawi, P. Vuorela, H. Vapaatalo, E. Moilanen:
Effects of flavonoids on prostaglandin E2 production, and on COX-2 and mPGES-1
expressions in activated macrophages.
Planta Med 77:1504-1511, 2011.

P. Kylli, L. Nohynek, R. Puupponen-Pimiä, B. Westerlund-Wikström, T. Leppänen, J.
Welling, E. Moilanen, M.I. Heinonen: Lingonberry (Vaccinium vitis-idaea) and European
cranberry (Vaccinium microcarpon) proanthocyanidins: isolation, identification, and
J Agr Food Chem 59:3373-3384, 2011.

M. Hämäläinen, R. Nieminen, P. Vuorela, M. Heinonen, E. Moilanen: The anti-
inflammatory effects of flavonoids: Genistein, kaempferol, quercetin and daidzein
inhibit STAT-1 and NF- B activation whereas flavone, isorhamnetin, naringenin and
pelargonidin inhibit only NF- B activation along with their inhibitory effect on iNOS
expression and NO production in activated macrophages.
Mediat Inflamm 2007:45673,2007.

S. Vuorela, K. Kreander, M. Karonen, R. Nieminen, M. Hämäläinen, A. Galkin, J.-P.
Salminen, E. Moilanen, K. Pihlaja, H. Vuorela , P. Vuorela, M. Heinonen: Preclinic
evaluation of rapeseed, raspberry and pine bark phenolics for health related effects.
J Agric Food Chem 53:5922-5931, 2005.

M. Karonen, M. Hämäläinen, R. Nieminen, K. D. Klika, J. Loponen,V.V. Ovcharenko, E.
Moilanen, K. Pihlaja: Phenolic Extractives from the Bark of Pinus sylvestris L. and their
Effects on Inflammatory Mediators Nitric Oxide and Prostaglandin E2.
J Agric FoodChem 52:7532-7540, 2004.